Differences in leaf anatomy determines temperature response of leaf hydraulic and mesophyll CO ₂ conductance in phylogenetically related C ₄ and C ₃ grass species

Leaf hydraulic and mesophyll CO2 conductance are both influenced by leaf anatomical traits, however it is poorly understood how the temperature response of these conductances differs between C4 C3 species with distinct anatomy. This study investigated (Kleaf), stomatal (gs) (gm) to CO2, traits in phylogenetically related Panicum antidotale (C4) P. bisulcatum (C3) grasses. The had lower outside xylem (Kox) Kleaf compared species. However, higher gm Traits associated water movement, Kox, increased more than species, whereas carbon uptake, Anet gm, Our findings demonstrate that, addition a concentrating mechanism, outside-xylem anatomy favours movement through stomata that provides an additional advantage for greater uptake relative loss increasing bisulcatum.